Other phenotypes associated with the sis2Δ mutant. (A and B) Abundance of several metabolites in the coenzyme A biosynthesis pathway as determined by targeted metabolomics on undiluted (A) or 10-fold diluted (B) extracts of strains without the reporter. Three biological replicates per strain. q-values are adjusted P-values (Materials and methods). Error bars show 2× SEM. (C) Growth tests on SC, pH 4.5 (left), or SC + 300 mM LiCl, pH 4.5 (right), for the indicated strains. Both plates are imaged after 2 days. (D) Culture doubling times of WT and sis2Δ in various environments. Data are from two experiments for each nitrogen condition. (E) vSEP ratio as a function of external potassium concentration. (F) pH-dependent and (G) pH-independent signals as a function of external potassium. Data are from two experiments. Biological triplicate measurements of two clones for each genotype were made, for a total of n = 6 replicates per genotype per experiment. Error bars show 95% confidence intervals.