Increased lifespan and MMP of the sis2Δ mutant are independent of fob1Δ and are blocked in a ppz1Δ background. (A) Kaplan–Meier lifespan estimates showing the interaction between sis2Δ and fob1Δ. Data are from two experiments. n = 1,131 for WT, n = 1,123 for sis2Δ, n = 1,157 for fob1Δ, and n = 1,139 for sis2Δfob1Δ. Shaded area is the 95% confidence interval. (B) Logarithm of the hazard ratio (“log[HR]”) calculated using CPH modeling (see Materials and methods). The filled square indicates the log(HR) was significantly different from zero (at an adjusted P-value of 0.05); empty square indicates the comparison was not significantly different from zero. Error bars show 95% confidence intervals. (C) MMP ratio trajectories. WT data are averaged over 17 experiments (n = 15,610); sis2Δ data are averaged over four experiments (n = 5,111); fob1Δ data are averaged over eight experiments (n = 6,144). Error bars show two times the SEM. (D) Mitochondrial OCR as measured using Seahorse (Materials and methods). Data are a representative experiment of three independent experiments. n = 4 replicates per genotype per experiment. Error bars show 2 × SEM. (E) Kaplan–Meier lifespan estimates showing the interaction between sis2Δ and ppz1Δ. Data are from four experiments. n = 2,356 for WT, n = 2,237 for sis2Δ, n = 3,015 for ppz1Δ, n = 2,282 for sis2Δppz1Δ. (F) log(HR) from a CPH model of E. (G) MMP as measured by TMRM for the strains in E, normalized to the mean WT value. Data are from two experiments. In each experiment, three replicate measurements of 10,000 cells were made for each strain. Error bars show 95% confidence intervals. CPH, Cox proportional hazard; SEM, standard error of the mean.