Characterization of the MMP reporter system. (A) Number of bud scars as a function of their WGA intensity (3 experiments, n = 2,576 cells). Error bars show 1.5 times the interquartile range. (B) Intensity of the MMP_s reporter component (green) and TMRM (magenta) measured in the same cells as a function of their age as determined by the number of bud scars. Data are normalized to the maximum value for each fluorescent marker so that the decline can be more easily compared. (C) Lifespans of strain with the full reporter system (green, 44 experiments, n = 44,618 cells) and without it (orange, 11 experiments, n = 17,099 cells). For B and C, error bars show 95% CI.