Figure S1.
Characterization of purified recombinant toxin and cryo-EM analysis with HL-60 cells and α-HL–mediated effect on RBCs. (A) Size-exclusion chromatography profile of purified α-HL monomer. (B) SDS-PAGE of SEC-purified recombinant α-HL monomer. (C) Data processing pipeline of α-HL in the presence of HL-60 cells. (D) Toxin-incubated RBC sample was imaged under a confocal microscope at different time points: (i) just after toxin addition; (ii) starting point of membrane damage after 2 min; (iii) lysis initiation state after 5 min; and (iv) lysed cells after 10 min. The cell membranes (in red color) were stained using Nile red dye (λexcitation = 540 nm, λemission = 565–610 nm). (E) Control RBC. (F) Bright-field imaging of toxin-treated RBC showing protrusion from the membrane. Source data are available for this figure: SourceData FS1.

Characterization of purified recombinant toxin and cryo-EM analysis with HL-60 cells and α-HL–mediated effect on RBCs. (A) Size-exclusion chromatography profile of purified α-HL monomer. (B) SDS-PAGE of SEC-purified recombinant α-HL monomer. (C) Data processing pipeline of α-HL in the presence of HL-60 cells. (D) Toxin-incubated RBC sample was imaged under a confocal microscope at different time points: (i) just after toxin addition; (ii) starting point of membrane damage after 2 min; (iii) lysis initiation state after 5 min; and (iv) lysed cells after 10 min. The cell membranes (in red color) were stained using Nile red dye (λexcitation = 540 nm, λemission = 565–610 nm). (E) Control RBC. (F) Bright-field imaging of toxin-treated RBC showing protrusion from the membrane. Source data are available for this figure: SourceData FS1.

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