Molecular analyses of Krt14 ^C373A/null transgenic mouse skin (complement to Fig. 7 ). (A and A′) K14 and (A′) K15 dot blots of whole tail skin lysate from 8-wk-old male Krt14^C373A/WT and Krt14^C373A/null littermates. For K14 immunoblotting, 7.5, 5, 2.5, 1, and 0.5 µg of lysate were loaded onto a membrane. For K15 immunoblotting, 5, 2.5, 1, 0.75, and 0.5 µg of lysate were loaded onto a membrane. Histone H3 was utilized as a loading control. Mean of K14 and K15 dot-blot MGV was normalized to histone H3. Comparisons were made using Mann–Whitney tests. **P = 0.0025. (B) Growth curve of mean weight of male and female Krt14^C373A/WT and Krt14^C373A/null littermates. Error bars represent SD. (C) Transcription of a YAP1 target gene, Cyr61, as measured via RT-qPCR in whole tail skin lysate from 8-wk-old male and female Krt14^C373A/WT and Krt14^C373A/null littermates. Dots represent three biological replicates. Comparisons were made using Mann–Whitney tests. *P < 0.005.