Analyses of keratin and 14-3-3 protein interactions (complement to Fig. 6 ). (A–C′) Lollipop plots displaying the 14-3-3-Pred “consensus score” for all serines and threonines in (A) human KRT14; (A′) mouse Krt14; (B) human KRT5; (B′) mouse Krt5; (C) human KRT15; (C′) mouse Krt15. Lollipop height and color both scale to consensus score magnitude. The two residues with the largest positive consensus score are labeled. (D) Lollipop plot illustrates identified phosphorylation sites on K14 protein exhibiting a localization score >0.6 in human N-TERT keratinocytes in culture. The relative occupancy percentage for each phosphorylation site was calculated by dividing the phosphopeptide’s intensity by total intensity of unique K14 peptides. This calculation, shown here as relative phosphorylation site occupancy (%), was performed only for phosphopeptides that are unique to K14 without miscleavages (S44, S281, S435, S437). (D′) Spectra of the phosphopeptides corresponding to the S39 (left) and S44 (right) sites. “Phos” conveys phosphorylation, and “CAM” stands for carbamidomethyl. (E) Representative micrographs of YAP1-14-3-3σ PLA performed on transfected HeLa cells. Cells were transfected with untagged K5 and WT and mutant K14 or K15. Merge panels show PLA punctae (red), EGFP-tagged keratin autofluorescence (green), and DAPI counterstain (blue). Scale bar = 10 µm.