Stutter cysteine is sufficient to relocalize YAP1 in transfected HeLa cells. (A and B) Representative images and magnified insets of HeLa cells transfected with mCherry-K5, EGFP-K14, and untagged WT and mutant K15. The mCherry signal is autofluorescence, and YAP1 is visualized through indirect immunostaining. Yellow dashed lines outline nuclei, and green dashed line outline the cell peripheries. Scale bar, 10 µm. (C) Scatter plots representing three pooled independent replicates, displaying mean and standard deviation. Dots represent a sc. Comparisons were made using Mann–Whitney tests. ****P < 0.0001, ***P = 0.0002 and 0.0003, **P = 0.0083.