Figure S5.
Giant Rab7-positive vesicles are proteolytically active compartments. (A) HEK-TDP-35-GFP cells were grown in media containing solvent control (DMSO; top row) or 100 μM CHQ for 18 h (next 2 rows, to show phenotypic diversity) before being fixed and immunostained for Rab7. Arrowheads indicate TDP-35 within Rab7-positive vesicles. N = 3; scale bar, 10 µm. (B) HEK-TDP-43-GFP and HEK-TDP-35-GFP cells were transfected with NEDD4-mCherry plasmid and grown in normal conditions for 24 h. Cells were then treated with 100 µM CHQ and incubated for 18 h before being fixed and immunostained for Rab7. Arrowheads indicate TDP-43 and TDP-35-GFP signals colocalized with NEDD4-mCherry (pseudocolored blue) within Rab7-positive giant vesicles. N = 3; scale bar, 10 µm.

Giant Rab7-positive vesicles are proteolytically active compartments. (A) HEK-TDP-35-GFP cells were grown in media containing solvent control (DMSO; top row) or 100 μM CHQ for 18 h (next 2 rows, to show phenotypic diversity) before being fixed and immunostained for Rab7. Arrowheads indicate TDP-35 within Rab7-positive vesicles. N = 3; scale bar, 10 µm. (B) HEK-TDP-43-GFP and HEK-TDP-35-GFP cells were transfected with NEDD4-mCherry plasmid and grown in normal conditions for 24 h. Cells were then treated with 100 µM CHQ and incubated for 18 h before being fixed and immunostained for Rab7. Arrowheads indicate TDP-43 and TDP-35-GFP signals colocalized with NEDD4-mCherry (pseudocolored blue) within Rab7-positive giant vesicles. N = 3; scale bar, 10 µm.

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