Figure 8.
Giant Rab7-positive vesicles are proteolytically active compartments. (A) HEK-TDP-43-GFP cells were grown in media containing solvent control (DMSO) or 100 μM CHQ for 18 h before being fixed and immunostained for Rab7. (B)N = 3; scale bar, 10 µm (B) HEK-TDP-43-GFP cells were transfected with siRNAs targeting NEDD4 or noncoding control and grown in normal conditions for 48 h. Cells were then treated with solvent control (DMSO) or 100 μM CHQ for 18 h before being fixed and immunostained for Rab7. (C)N = 3; scale bar, 10 µm (C) Quantification of data from B showing percentage of cells with enriched TDP-43-GFP signal within Rab7-stained MVBs. ***, P < 0.001 by one-way analysis of variance with Tukey’s post hoc test; N = 3. Error bars represent standard error of the mean. Scale bar, 10 µm.

Giant Rab7-positive vesicles are proteolytically active compartments. (A) HEK-TDP-43-GFP cells were grown in media containing solvent control (DMSO) or 100 μM CHQ for 18 h before being fixed and immunostained for Rab7. (B)N = 3; scale bar, 10 µm (B) HEK-TDP-43-GFP cells were transfected with siRNAs targeting NEDD4 or noncoding control and grown in normal conditions for 48 h. Cells were then treated with solvent control (DMSO) or 100 μM CHQ for 18 h before being fixed and immunostained for Rab7. (C)N = 3; scale bar, 10 µm (C) Quantification of data from B showing percentage of cells with enriched TDP-43-GFP signal within Rab7-stained MVBs. ***, P < 0.001 by one-way analysis of variance with Tukey’s post hoc test; N = 3. Error bars represent standard error of the mean. Scale bar, 10 µm.

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