NEDD4 knockdown stabilizes TDP-43 in iNeurons. (A) Schematic of optical pulse labeling strategy for measuring TDP-43-Dendra2 half-life. (B) NEDD4 knockdown efficiency (lentiviral shRNA), based on NEDD4 image intensity (NT = nontargeting) *, P < 0.05 by Welch’s two-tailed T test. (C) TDP-43-Dendra2 half-life in iNeurons with or without NEDD4 depletion. P value determined using sum of squares F test. Error bars in all graphical panels represent standard error of the mean.