HEK293A cells were transfected with HA-ubiquitin (pRB506) or both HA-Ub and NEDD4-mCherry (pRB334) and grown for 2 days before TDP-43 was immunoprecipitated. (A) Representative biological replicate of K63 and K48 Ub signal, NEDD4, TDP-43, and α-tubulin from a single biological replicate; run on a 7% SDS-PAGE gel. (B) As in A, except Pan-Ub signal is shown in place of K63 and K48 on a gradient gel. Arrowheads indicate full-length species of TDP-43 and NEDD4-mCherry, with presumed degradation products/protein isoforms shown below. Trends shown were reproduced across five biological replicates. Source data are available for this figure: SourceData FS2.