Knockout of the Rsp5 adaptor Rim8 increases TDP-43-YFP levels. Indicated strains were transformed with TDP-43-YFP plasmid (pRB109) and grown to mid-logarithmic growth phase. TDP-43-YFP levels were detected by western blot using anti–TDP-43 antibody and normalized to GAPDH loading control; quantification is shown here. *, P < 0.05; by one-way analysis of variance with Tukey’s post hoc test; N = 3, except for strains art2-4Δ, art8Δ, art10Δ, bsd2Δ, sna3Δ, and bul1-2Δ (N = 2). Error bars represent standard error of the mean.