Figure 1.
A dot-blot genetic screen in yeast identifies genes regulating TDP-43 steady-state levels. (A) Schematic representation of the dot-blot genetic screen. (B) Example dot-blot with control strains and placement indicated. (C) Gene ontology terms of biological functions significantly enriched within the hits from the dot-blot genetic screen. (D) Indicated strains were transformed with TDP-43-mRuby2 plasmid (pRB194) and grown to mid-logarithmic growth phase, and TDP-43-mRuby2 was detected by western blot using anti–TDP-43 antibody. TDP-43 levels were normalized to GAPDH loading control. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way analysis of variance with Tukey’s post hoc test; N = 3. Error bars indicate standard error of the mean. Source data are available for this figure: SourceData F1.

A dot-blot genetic screen in yeast identifies genes regulating TDP-43 steady-state levels. (A) Schematic representation of the dot-blot genetic screen. (B) Example dot-blot with control strains and placement indicated. (C) Gene ontology terms of biological functions significantly enriched within the hits from the dot-blot genetic screen. (D) Indicated strains were transformed with TDP-43-mRuby2 plasmid (pRB194) and grown to mid-logarithmic growth phase, and TDP-43-mRuby2 was detected by western blot using anti–TDP-43 antibody. TDP-43 levels were normalized to GAPDH loading control. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way analysis of variance with Tukey’s post hoc test; N = 3. Error bars indicate standard error of the mean. Source data are available for this figure: SourceData F1.

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