Figure 3.
Rab1A-GTP binds directly to CALCOCO1 and OPTN. (A and B) Coomassie gels showing in vitro binding to GST-Rab1A–coated beads of either purified GFP-CALCOCO1 (A) or GFP-OPTN (B), with GFP as a negative control. Rab1A was in GTP- or GDP-locked forms as indicated. (C and D) GUV-binding assay using GTP- or GDP-locked Rab1A on the GUV with applied GFP-CALCOCO1 (C) or GFP-OPTN (D). Each large datapoint in the graph depicts the average mean fluorescence intensity of GFP-CALCOCO1 or GFP-OPTN on a selection of GUV membrane and represents an independent experiment (n = 3), with smaller gray datapoints representing all the technical replicates (AU, arbitrary units). The mean ± SD is indicated. **P < 0.01 (unpaired t test). Source data are available for this figure: SourceData F3.

Rab1A-GTP binds directly to CALCOCO1 and OPTN. (A and B) Coomassie gels showing in vitro binding to GST-Rab1A–coated beads of either purified GFP-CALCOCO1 (A) or GFP-OPTN (B), with GFP as a negative control. Rab1A was in GTP- or GDP-locked forms as indicated. (C and D) GUV-binding assay using GTP- or GDP-locked Rab1A on the GUV with applied GFP-CALCOCO1 (C) or GFP-OPTN (D). Each large datapoint in the graph depicts the average mean fluorescence intensity of GFP-CALCOCO1 or GFP-OPTN on a selection of GUV membrane and represents an independent experiment (n = 3), with smaller gray datapoints representing all the technical replicates (AU, arbitrary units). The mean ± SD is indicated. **P < 0.01 (unpaired t test). Source data are available for this figure: SourceData F3.

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