Axonal transport speeds calculated from data in Fig. 3 , the defect in lysosomal transport of spin-GFP positive lysosomes in Rab2 null larvae, and apparently normal axonal DCV transport in prd1 null larvae. (A and B) Anterograde (A) and retrograde (B) DCV movement speeds from the experiments in Fig. 3 (OK6 > ILP2-GFP). Number of larvae analyzed: control 29, Rab2 10, syd^z4/Df 12, Dhc-KD 12, Khc-KD 8, Arl8/Df 9, and unc-104P350/O3.1 12. (C) Representative kymographs showing transport of Spinster-positive organelles in motor axons (OK6 > Spinster-GFP) in control and Rab2 larvae. Scale bar: 10 µm. (D) Left, directional distributions derived from C, averaged from six control and six Rab2 larvae. Right, the logarithmic ratio of retrograde to anterograde peak amplitude and the relative static peak amplitude for the directional distributions at the left. (E) Representative kymograph showing DCV transport in motor axons of prd1^M56/Df larvae. Scale bar: 10 µm. (F) Directional distribution derived from E, averaged from four prd1^M56/Df larvae (blue curve), shown together with a replica of the directional distribution of control larvae in Fig. 3, B–D (gray curve). Arl8/Df results in A and B, and the results in C and D represent reanalysis of data published earlier (Lund et al., 2021). Bar graphs in A, B, and D, right represent mean + SEM. ANOVA followed by Dunnett’s test (A), Steel with control test (B), Student’s t test (D, right).