Ecdysone regulates the loss of apical-basal cell polarity during FBR. (A–E′) Confocal images of fat body from CAAX-GFP–expressing, 3 h APF pupae immunostained for aPKC (A), Par-6 (B), Crumbs (C), Baz (D), and Dlg (E), imaged on sides a (top) and b (bottom), shown in lateral view. Quantification of mean intensities of aPKC (A′), Par-6 (B′), Crumbs (C′), Baz (D′), and Dlg (E′; at the surfaces, paired for each fat body tissue, n: 10 tissues, 3 surface ROIs per tissue per side). Paired two-sided t test, ns P > 0.05. (F–K) Confocal images of fat body from 3 h APF pupae expressing Lsp2-Gal4 +control or +UAS-EcRDN⁶⁸⁶⁹ (F, G, and H, left and right, respectively) immunostained for Dlg (F), Baz (G), and Crumbs (H, side a [top] and b [bottom], lateral view, with blue and orange arrowheads pointing at cell surface or lateral domain, respectively). Quantification of mean intensities of Dlg (F′ and F″), Baz (G′ and G″), and Crumbs (H′ and H″) on surfaces on sides a and b for control or UAS-EcRDN⁶⁸⁶⁹ (mean of mean intensities from several ROIs, data paired by tissue; n: 10 tissues, 3 surface ROIs per tissue per side). Paired two-sided t test, ****P < 0.0001. Quantification of mean intensities of Dlg (I), Baz (J), and Crumbs (K) at the surface for control and UAS-EcRDN⁶⁸⁶⁹ on side b (I) or side a (J and K). Unpaired two-sided t test, ****P < 0.0001. Scale bars: 20 µm (A–E and F–H).