![aPKC restricts the localization of Crumbs, Baz, and Dlg in the fat body. (A–N″) Confocal images of larval fat body expressing Lpp2-Gal4+UAS-Myr-td-Tom +control, +UAS-aPKC RNAi34332, or +UAS-Crumbs RNAi39117 (A–C, left, middle and right, respectively) immunostained for Dlg (B) and Baz (C), expressing Lpp2-Gal4+UAS-Myr-td-Tom +control or +UAS-aPKC RNAi34332 (D–I, left and right, respectively) immunostained for Crumbs (E), E-Cad (G), or phospho-(PS980)-Baz (I), expressing Lpp2-Gal4+UAS-Myr-td-Tom +control or +UAS-Crumbs RNAi39117 (J and K, left and right, respectively) immunostained for aPKC (K) or expressing Lpp2-Gal4+UAS-Myr-td-Tom +control or +UAS-Scribble RNAi105412 (L–N, left and right, respectively) immunostained for aPKC (M) and Dlg (N; side a [top] and b [bottom] in lateral view). Quantification of mean intensities of Dlg (B′–B‴, N′ and N″), Baz (C′–C‴), Crumbs (E′ and E″), E-Cad (G′ and G″), phospho-Baz (I′ and I″), and aPKC (K′ and K″, M′ and M″) on surfaces on sides a and b for control (B′, C′, E′, G′, I′, K′, M′, and N′), UAS-aPKC RNAi34332 (B″, C″, E″, G″, and I″), UAS-Crumbs RNAi39117(B‴, C‴, and K″) or UAS-Scribble RNAi105412 (M″ and N″) or on lateral domain for phospho-Baz for control (I‴), UAS-aPKC RNAi34332 (I‴′; mean of mean intensities from several ROIs, data paired by tissue; n: six tissues, three surface or lateral ROIs per tissue per side). Paired two-sided t test, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, and ns P > 0.05. Scale bars: 20 µm (A–N).](https://cdn.rupress.org/rup/content_public/journal/jcb/225/3/10.1083_jcb.202504139/1/jcb_202504139_fig5.png?Expires=1771196086&Signature=jVVxaFjwnTdbUUqsg6cDHGz5Jdp8Yd9NXRTyZXJzgSHVffo6XB9sqZ0q1wv1ruXa2rkcoI9-etg1Rx9nZ2Z15pUcDx~Y2gfDqPHgNYBvXXYQ8eKT549NQjGreEmq3G3XGu3IKLQ2N3xpPJidpLOOJl6NZU7Z3nKMdXQEaOyVFcwFo5DvZN-MAMLybsOWb~RhzS3mPt~mw2pcCxhfKa6HHJWqUrayb8FV9I8FAYMSsjOdsfXCGSWT3pvxYKlugq~uavggB4lFuEZSwyjx82hJVYJWGljsvhvR1GY6LQUvXADx6DIytx7VrGVyjuDQn~vaSzt63nd7peN4SFNavVO3uQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
aPKC restricts the localization of Crumbs, Baz, and Dlg in the fat body. (A–N″) Confocal images of larval fat body expressing Lpp2-Gal4+UAS-Myr-td-Tom +control, +UAS-aPKC RNAi34332, or +UAS-Crumbs RNAi39117 (A–C, left, middle and right, respectively) immunostained for Dlg (B) and Baz (C), expressing Lpp2-Gal4+UAS-Myr-td-Tom +control or +UAS-aPKC RNAi34332 (D–I, left and right, respectively) immunostained for Crumbs (E), E-Cad (G), or phospho-(PS980)-Baz (I), expressing Lpp2-Gal4+UAS-Myr-td-Tom +control or +UAS-Crumbs RNAi39117 (J and K, left and right, respectively) immunostained for aPKC (K) or expressing Lpp2-Gal4+UAS-Myr-td-Tom +control or +UAS-Scribble RNAi105412 (L–N, left and right, respectively) immunostained for aPKC (M) and Dlg (N; side a [top] and b [bottom] in lateral view). Quantification of mean intensities of Dlg (B′–B‴, N′ and N″), Baz (C′–C‴), Crumbs (E′ and E″), E-Cad (G′ and G″), phospho-Baz (I′ and I″), and aPKC (K′ and K″, M′ and M″) on surfaces on sides a and b for control (B′, C′, E′, G′, I′, K′, M′, and N′), UAS-aPKC RNAi34332 (B″, C″, E″, G″, and I″), UAS-Crumbs RNAi39117(B‴, C‴, and K″) or UAS-Scribble RNAi105412 (M″ and N″) or on lateral domain for phospho-Baz for control (I‴), UAS-aPKC RNAi34332 (I‴′; mean of mean intensities from several ROIs, data paired by tissue; n: six tissues, three surface or lateral ROIs per tissue per side). Paired two-sided t test, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, and ns P > 0.05. Scale bars: 20 µm (A–N).