Figure S3.
Apical-basal cell polarity is needed for collagen-IV–dependent cell–cell adhesion—related to Fig. 2, A–D. (A–F) Confocal single Z plane images of larval fat body expressing Lpp-Gal4+UAS-Myr-td-Tomato +control (A), UAS-aPKC RNAi105624 (B), UAS-Crumbs RNAi34999 (C), UAS-Crumbs RNAi330135 (D), UAS-Scribble RNAi35748 (E), or UAS-Lgl RNAi109604 (D; yellow or white arrow showing gaps at tricellular or bicellular vertices, respectively). (G–N″) Confocal single Z plane images of larval fat body expressing Lpp-Gal4+UAS-Myr-td-Tomato +control (G), UAS-Mys RNAi27735 (H), UAS-E-Cad RNAi32904 (I), UAS-Viking RNAi106812 (J), or UAS-Viking RNAi106812+UAS-E-Cad RNAi32904 (K; yellow or white arrow showing gaps at tricellular or bicellular vertices, respectively). Quantification of percentage of tricellular or bicellular cell–cell vertices containing gaps per image (L, M, respectively) from G–K (n: 12 images for each genotype, one image from each side from 6 different larvae). Kruskal–Wallis test followed by Dunn’s multiple comparisons, ****P < 0.0001, **P < 0.01, and ns P > 0.05. Confocal images of larval fat bodies expressing Lpp-Gal4+UAS-Myr-td-Tomato +control or UAS-Mys RNAi27735 (N, top and bottom, respectively) immunostained for Mys (showing Mys on sides a and b in lateral views). Quantification of mean intensity of Mys for control (N′) and UAS-Mys RNAi27735 (N″) on surfaces on sides a and b (mean of mean intensities from several ROIs, data paired by tissue; n: six tissues, three surface ROIs per side). Unpaired two-sided t test, ****P < 0.0001. (O–V) Confocal single Z plane images of larval fat body expressing Lpp-Gal4+UAS-Myr-td-Tomato +control (O), UAS-E-Cad RNAi103962 (P), UAS-aPKC RNAi34332 (Q), UAS-E-Cad RNAi103962+UAS-aPKC RNAi34332 (R), UAS-Scribble RNAi105412 (S), or UAS-aPKC RNAi34332+UAS-Scribble RNAi105412 (T; yellow or white arrow showing gaps at tricellular or bicellular vertices, respectively). Quantification of percentage of tricellular or bicellular cell–cell vertices containing gaps per image (U, V, respectively) from O–T (n: 12 images for control, E-Cad RNAi103962, Scribble RNAi105412, and aPKC RNAi34332+Scribble RNAi105412 one image from each side from six different larvae and 36 images for aPKC RNAi34332 and E-Cad RNAi103962+aPKC RNAi34332 from six different larvae, half of which from each side). Kruskal–Wallis test followed by Dunn’s multiple comparisons, ****P < 0.0001, ***P < 0.001, **P < 0.01, and ns P > 0.05. Scale bars: 20 µm (A–F, G–K, and O–T).

Apical-basal cell polarity is needed for collagen-IV–dependent cell–cell adhesion—related to Fig. 2, A–D. (A–F) Confocal single Z plane images of larval fat body expressing Lpp-Gal4+UAS-Myr-td-Tomato +control (A), UAS-aPKC RNAi105624 (B), UAS-Crumbs RNAi34999 (C), UAS-Crumbs RNAi330135 (D), UAS-Scribble RNAi35748 (E), or UAS-Lgl RNAi109604 (D; yellow or white arrow showing gaps at tricellular or bicellular vertices, respectively). (G–N″) Confocal single Z plane images of larval fat body expressing Lpp-Gal4+UAS-Myr-td-Tomato +control (G), UAS-Mys RNAi27735 (H), UAS-E-Cad RNAi32904 (I), UAS-Viking RNAi106812 (J), or UAS-Viking RNAi106812+UAS-E-Cad RNAi32904 (K; yellow or white arrow showing gaps at tricellular or bicellular vertices, respectively). Quantification of percentage of tricellular or bicellular cell–cell vertices containing gaps per image (L, M, respectively) from G–K (n: 12 images for each genotype, one image from each side from 6 different larvae). Kruskal–Wallis test followed by Dunn’s multiple comparisons, ****P < 0.0001, **P < 0.01, and ns P > 0.05. Confocal images of larval fat bodies expressing Lpp-Gal4+UAS-Myr-td-Tomato +control or UAS-Mys RNAi27735 (N, top and bottom, respectively) immunostained for Mys (showing Mys on sides a and b in lateral views). Quantification of mean intensity of Mys for control (N′) and UAS-Mys RNAi27735 (N″) on surfaces on sides a and b (mean of mean intensities from several ROIs, data paired by tissue; n: six tissues, three surface ROIs per side). Unpaired two-sided t test, ****P < 0.0001. (O–V) Confocal single Z plane images of larval fat body expressing Lpp-Gal4+UAS-Myr-td-Tomato +control (O), UAS-E-Cad RNAi103962 (P), UAS-aPKC RNAi34332 (Q), UAS-E-Cad RNAi103962+UAS-aPKC RNAi34332 (R), UAS-Scribble RNAi105412 (S), or UAS-aPKC RNAi34332+UAS-Scribble RNAi105412 (T; yellow or white arrow showing gaps at tricellular or bicellular vertices, respectively). Quantification of percentage of tricellular or bicellular cell–cell vertices containing gaps per image (U, V, respectively) from O–T (n: 12 images for control, E-Cad RNAi103962, Scribble RNAi105412, and aPKC RNAi34332+Scribble RNAi105412 one image from each side from six different larvae and 36 images for aPKC RNAi34332 and E-Cad RNAi103962+aPKC RNAi34332 from six different larvae, half of which from each side). Kruskal–Wallis test followed by Dunn’s multiple comparisons, ****P < 0.0001, ***P < 0.001, **P < 0.01, and ns P > 0.05. Scale bars: 20 µm (A–F, G–K, and O–T).

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