E-cadherin RNAi is not sufficient to induce cell–cell dissociation in larval fat body. (A–C′) Confocal images of larval fat body expressing Lpp-Gal4+UAS-Myr-td-Tomato +control (A), +UAS-E-cadherin RNAi¹⁰³⁹⁶² (B), or +UAS-E-cadherin RNAi²⁷⁰⁸² (C) immunostained for E-cadherin (sides a and b shown in planar [top] and lateral views [bottom]). Related to Fig. 3, C–E. Note that the same control images are displayed in Fig. 3 C and Fig. S2 A. Fig. 3, C–E and Fig. S2, A–C′ are the results from the same experiment and hence the control is the same for both. Quantification of mean intensity of E-cadherin for control (A′), UAS-E-cadherin RNAi¹⁰³⁹⁶² (B′), or UAS-E-cadherin RNAi²⁷⁰⁸² (C′) on surfaces on sides a and b (mean of mean intensities from several ROIs, data paired by tissue; n: three tissues, three surface ROIs per side). Unpaired two-sided t test, **P < 0.01, ns P > 0.05. Scale bars: 20 µm (A–C).