Figure S1.
The larval fat body tissue exhibits apical-basal cell polarity. (A and B) Confocal images of larval fat bodies expressing Lpp-Gal4+UAS-Myr-td-Tom (A) or Lgl-GFP (B; imaged across the whole tissue from side a [top] toward side b [bottom] [A] or from side b [bottom] toward side a [top] [A′ and B] shown in lateral view, arrow indicates direction of imaging). (C–F″) Confocal images of CAAX-GFP–expressing larval fat body immunostained for aPKC (C), Par-6 (D), Crumbs (E), and Dlg (F; imaged separately starting from side a [top] or b [bottom], merged channels shown in lateral view, CAAX-GFP in green and antibody stain in magenta). Quantification of mean intensities of CAAX-GFP on surface ROIs (‘, yellow background) or lateral ROIs (‘‘, orange background) on sides a and b (mean of mean intensities from several ROIs at the surface or lateral domain of same tissue, data paired by tissue, quantifications using same samples as in Fig. 1, B–E; n: 10 tissues, 3 surface or lateral ROIs per side (Fig. 1, B′–E′ and B″–E″). Paired two-sided t test, ns P > 0.05. Related to Fig. 1, B–E. (G–I′) Confocal images of larval fat bodies expressing Lpp-Gal4+UAS-Myr-td-Tom with Phalloidin-488 staining to label actin (G) or expressing Lpp-Gal4+UAS-Myr-td-Tom+UAS-GFP-Golgi (H) or expressing Laminin-B1-GFP with DAPI staining (I), imaged on sides a (top) and b (bottom), shown in lateral view. Quantification of mean intensities of Phalloidin (G′) or GFP-Golgi (H′; in ROI proximal to the cell surfaces, paired for each fat body tissue, n: six tissues, three surface ROIs per tissue per side) or of distance of nuclear surface from cell surface (I′, paired for each fat body tissue, n: six tissues, three images per side). Paired two-sided t test, ns P > 0.05. (J–K′) Confocal images of Venus-Ilk–expressing (J) or If-YFP–expressing (K) larval fat body (side a [top] and b [bottom], lateral view). Quantification of mean intensity of Venus-Ilk (J′) and If-YFP (K′) on surfaces on sides a and b (mean of mean intensities from several ROIs, data paired by tissue; n: six tissues, three surface ROIs per side). Unpaired two-sided t test, ****P < 0.0001. Related to Fig. 2. Scale bars: 20 µm (A–K).

The larval fat body tissue exhibits apical-basal cell polarity. (A and B) Confocal images of larval fat bodies expressing Lpp-Gal4+UAS-Myr-td-Tom (A) or Lgl-GFP (B; imaged across the whole tissue from side a [top] toward side b [bottom] [A] or from side b [bottom] toward side a [top] [A′ and B] shown in lateral view, arrow indicates direction of imaging). (C–F″) Confocal images of CAAX-GFP–expressing larval fat body immunostained for aPKC (C), Par-6 (D), Crumbs (E), and Dlg (F; imaged separately starting from side a [top] or b [bottom], merged channels shown in lateral view, CAAX-GFP in green and antibody stain in magenta). Quantification of mean intensities of CAAX-GFP on surface ROIs (‘, yellow background) or lateral ROIs (‘‘, orange background) on sides a and b (mean of mean intensities from several ROIs at the surface or lateral domain of same tissue, data paired by tissue, quantifications using same samples as in Fig. 1, B–E; n: 10 tissues, 3 surface or lateral ROIs per side (Fig. 1, B′–E′ and B″–E″). Paired two-sided t test, ns P > 0.05. Related to Fig. 1, B–E. (G–I′) Confocal images of larval fat bodies expressing Lpp-Gal4+UAS-Myr-td-Tom with Phalloidin-488 staining to label actin (G) or expressing Lpp-Gal4+UAS-Myr-td-Tom+UAS-GFP-Golgi (H) or expressing Laminin-B1-GFP with DAPI staining (I), imaged on sides a (top) and b (bottom), shown in lateral view. Quantification of mean intensities of Phalloidin (G′) or GFP-Golgi (H′; in ROI proximal to the cell surfaces, paired for each fat body tissue, n: six tissues, three surface ROIs per tissue per side) or of distance of nuclear surface from cell surface (I′, paired for each fat body tissue, n: six tissues, three images per side). Paired two-sided t test, ns P > 0.05. (J–K′) Confocal images of Venus-Ilk–expressing (J) or If-YFP–expressing (K) larval fat body (side a [top] and b [bottom], lateral view). Quantification of mean intensity of Venus-Ilk (J′) and If-YFP (K′) on surfaces on sides a and b (mean of mean intensities from several ROIs, data paired by tissue; n: six tissues, three surface ROIs per side). Unpaired two-sided t test, ****P < 0.0001. Related to Fig. 2. Scale bars: 20 µm (A–K).

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