Deconvolution of bulk human LN dataset and fibroblast in spatial and single-cell dataset. (A) Dot plot showing distribution of cell type proportion from deconvolution results on each bulk RNA-seq human LN TB granuloma sample, separated by cell type and colored by TB conditions. Only cell types with significant difference between TB conditions are shown. Two-sided T test with Bonferroni correction was used to compare the means. Statistical annotations: P value < 0.05 (*) and P value < 0.01 (**). (B) Cell2loc imputed fibroblast abundance distribution on the Visium dataset group by TB and granuloma status (Materials and methods). The 5% quantile of the estimated posterior distribution of cell abundance per Visium spot is displayed, representing the value of cell abundance that the model has high confidence in. Two-sided Mann–Whitney U test without correction were used for statistical testing. P value < 0.0001 (****); P value > 0.05 (ns). (C) Same as B, but grouped by HIV and TB status. (D) Bar plot of patient distribution in each fibroblast subcluster. (E) UMAP embedding of fibroblasts colored by HIV status of the sample.