Lineage tracing and CLAUDIN 1 protein expression in thymic DC1s. (A) Schematic of the mouse model used for lineage tracing. (B) Representative flow cytometry plots show TdTOMATO expression within thymic DC subsets from XCR1^iCreR26^TdTOMATO mouse model. (C) Frequency of TdTOMATO⁺ cells within DC subsets from Fig. 2 B (mean ± SEM, n = 14 mice from three independent experiments). (D) Design of BrdU DC1 lineage tracing experiment. (E) Representative flow cytometry plots show the frequency of BrdU⁺ cells within DC1 subsets on indicated days after the BrdU administration related to Fig. 2 D. (F) Percentage of BrdU⁺ cells within DC1 lineage subsets on indicated days after BrdU administration related to Fig. 2 E (mean ± SEM, n = 3–5 mice from two independent experiments). (G) Representative flow cytometry plots show CLAUDIN 1 positivity within thymic DC1 subsets. FMO controls are shown. (H) Frequency of CLAUDIN 1⁺ cells and MFI of CLAUDIN 1 expression within thymic DC1 subsets related to Fig. 2 G (mean ± SEM, n = 20–35 mice from a minimum of three independent experiments). Statistical analysis in F was performed using RM one-way ANOVA with Tukey’s multiple comparisons test, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P < 0.0001, ns = not significant. All mice were bred on the B6 background. Littermates were used as controls. FMO, Fluorescence minus one.