Claudin 1 is upregulated in CAT-experienced DC1s. (A) FACS gating strategy used to perform scRNAseq of thymic myeloid cells. Thymic cells were isolated from Foxn1^CreR26^TdTOMATO mice, MACS-enriched for CD11c⁺ and CD11b⁺ cells, and sorted as either TdTOMATO⁺ or TdTOMATO−CD45⁺ CD11c⁺/CD11b⁺ cells. (B) UMAP of annotated thymic myeloid cells from scRNAseq excluding subsets shown in red in the legend of Fig. S1 B. Individual cell lineages are demarcated by dotted lines. DC = conventional DC; aDC = activated DC; Mac = macrophages; Mono = monocytes; prolif = proliferating. (C) UMAP of scRNAseq corresponding to Fig. 1 B projecting CAT-experienced (orange) and CAT-inexperienced (gray) cells. A dotted line shows the DC1 subset. (D) Heat map showing top 10 down- and upregulated genes in CAT-experienced over CAT-inexperienced DC1s. The heat map color scale depicts average log2 fold change. (E) Violin plots show the expression of Cldn1 and Cd36 by CAT-experienced (orange) and CAT-inexperienced (gray) DC1. All mice were bred on the B6 background. Littermates were used.