Figure S5.
4-OHT dosing selection for embryos and adults. (A) Schematic showing the 4-OHT treatment strategy on embryos. (B) Table showing survival rates of embryos treated with different concentrations of 4-OHT from the sphere stage to 2 dpf. (C) Gel electrophoretic image showing the T7E1 assay outcome of the tbx16 locus. The mutant band was indicated by the arrow. NC, negative control. (D) Immunostaining of Cas9 (magenta) and nuclei stained by DAPI (blue) in PGCs within the DMSO or 40 μM 4-OHT–treated embryos at 2 dpf. (E) Schematic showing the 4-OHT treatment strategy on 45 dpf females and detection of spawning rates. (F) Bar chart showing the spawning rates of adults treated with 10 and 5 μM 4-OHT at 45 dpf. Adults: The serial number of treated transgenic zebrafish. Source data are available for this figure: SourceData FS5.

4-OHT dosing selection for embryos and adults. (A) Schematic showing the 4-OHT treatment strategy on embryos. (B) Table showing survival rates of embryos treated with different concentrations of 4-OHT from the sphere stage to 2 dpf. (C) Gel electrophoretic image showing the T7E1 assay outcome of the tbx16 locus. The mutant band was indicated by the arrow. NC, negative control. (D) Immunostaining of Cas9 (magenta) and nuclei stained by DAPI (blue) in PGCs within the DMSO or 40 μM 4-OHT–treated embryos at 2 dpf. (E) Schematic showing the 4-OHT treatment strategy on 45 dpf females and detection of spawning rates. (F) Bar chart showing the spawning rates of adults treated with 10 and 5 μM 4-OHT at 45 dpf. Adults: The serial number of treated transgenic zebrafish. Source data are available for this figure: SourceData FS5.

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