Mutation efficiency of tbx16 alleles in PGCs. (A) Sanger sequencing peak maps showing different mutation types of tbx16 alleles (WT, heterozygous mutation, and homozygous mutation). The shadow yellow box indicates the tbx16 target site. (B) Allelic mutant types and ratios. Green, WT sequence; gray, UTRs; orange line, deleted region; blue, inserted region. (C) ISH-detected ddx4 expression in control and tbx16 PGC-KO embryos at 24 hpf. The box plot showing the ratios of normal and mislocated PGCs in control and PGC-KO embryos. Ne, number of embryos. Source data are available for this figure: SourceData FS4.