Off-target analysis of the tbxta locus with WGS and NGS. (A) Schematic showing PGC collection for WGS. (B) Bar chart showing the mutation rates of PGCs within 4-OHT– or DMSO-treated embryos at the tbxta locus. Red letters indicate the sequence of tbxta target sites. Target site, tbxta gRNA target site; R1 to R4, the predicted tbxta gRNA off-target sites. INDEL, insertion-deletion; REF, reference genome; SNV, single-nucleotide variant.