Figure S2.
GC specificity of the zebrafish piwil1 promoter. (A) Identification of a shorter piwil1 promoter with GC specificity. Left, Tol2 transposon–based construct structures; right, representative GFP expression patterns in zebrafish larvae after injection with corresponding constructs. GFP was observed under fluorescent dissection microscopy. Note that the 2.5-kb piwil1 promoter retained GC-specific transcription activity and was used subsequently. (B) A transgenic line with GC-specific expression of membrane-localizing mCherry driven by the 2.5-kb piwil1 promoter. CAAX, cell membrane localizing signal; n3U, 3′ UTR of the zebrafish nanos3 gene. Note that mCherry was ubiquitously expressed at the 512-cell stage but became restricted to PGCs thereafter. The boxed areas in the top panel were enlarged in the lower panel. Source data are available for this figure: SourceData FS2.

GC specificity of the zebrafish piwil1 promoter. (A) Identification of a shorter piwil1 promoter with GC specificity. Left, Tol2 transposon–based construct structures; right, representative GFP expression patterns in zebrafish larvae after injection with corresponding constructs. GFP was observed under fluorescent dissection microscopy. Note that the 2.5-kb piwil1 promoter retained GC-specific transcription activity and was used subsequently. (B) A transgenic line with GC-specific expression of membrane-localizing mCherry driven by the 2.5-kb piwil1 promoter. CAAX, cell membrane localizing signal; n3U, 3′ UTR of the zebrafish nanos3 gene. Note that mCherry was ubiquitously expressed at the 512-cell stage but became restricted to PGCs thereafter. The boxed areas in the top panel were enlarged in the lower panel. Source data are available for this figure: SourceData FS2.

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