Figure S1.
The nuclear translocation ability of Cas9 protein expressed in HEK293T cells. (A) General structure of transfection constructs. CMV, cytomegaloviral promoter; zCas9, Cas9 with codon optimization for the zebrafish; ERT2, human estrogen receptor with three missense mutations (G400V/M543A/L544A) in the ligand-binding domain; NLS1, SV40 nuclear localization signal; NLS2, nucleoplasmin NLS; pA, polyadenylation signal. (B) Nuclear translocation ability of different Cas9 fusion proteins with or without 4-OHT treatment. The transfected cells were immunostained with Cas9 antibody plus DAPI staining for nuclei 24 hpt. The top of each group of images showed the schematic structure of the Cas9 fusion protein. Source data are available for this figure: SourceData FS1.

The nuclear translocation ability of Cas9 protein expressed in HEK293T cells. (A) General structure of transfection constructs. CMV, cytomegaloviral promoter; zCas9, Cas9 with codon optimization for the zebrafish; ERT2, human estrogen receptor with three missense mutations (G400V/M543A/L544A) in the ligand-binding domain; NLS1, SV40 nuclear localization signal; NLS2, nucleoplasmin NLS; pA, polyadenylation signal. (B) Nuclear translocation ability of different Cas9 fusion proteins with or without 4-OHT treatment. The transfected cells were immunostained with Cas9 antibody plus DAPI staining for nuclei 24 hpt. The top of each group of images showed the schematic structure of the Cas9 fusion protein. Source data are available for this figure: SourceData FS1.

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