HIV-1 provirus expression and production of infectious virus particles after treatment treatment with the mSMAC AZD5582. (A) Frequency of cells expressing HIV-1 LTR, gag, and env transcripts after treatment with mSMAC estimated by poison distribution, based on limiting dilution data and calculated using the MPN method. (B) Average copies of transcripts per expressing cell. The number of LTR, gag, and env transcripts per reaction was determined based on a standard curve. Average copies of transcripts per expressing cell were estimated based on the frequency of cells expressing HIV-1 transcripts as shown in A. (C) Gag p24 protein detection by ELISA in the supernatant of healthy cells infected with supernatant from the HIV-1⁺ CD4⁺ T cell clones resting and after treatment with mSMAC, analyzed 5 days after infection.