CTLA-4 Y218* mutation leads to dysfunctional TE. (A–C) (A) Experimental design and (B) representative dot plot of CD80 donor levels following TE by Jurkat recipient cells with WT (blue blots) or Y218* CTLA-4 mutation (red blots), with relative ligand loss quantified in C using multiple unpaired T test. N = 6 independent repeats for each condition. ***P < 0.001; ns: not significant.