Detailed comparisons between nearest-neighbor states. (A) The region around the ligand Asp, β1-α1 loop, α1-helix, and β6-α7 loop where movement is greatest between states 1 and 6 (S1–S6). (B) The region around the α1 helix, α1′ helix, β6-α7 loop, and α7 helix where movement is greatest between states 6 and 8. Each panel compares two nearest-neighbor states. For economy, and to compare the two rotamers of W129 in states 1–5, image 1 in B compares states 4 and 6. The carbons and metal ions of each state are in the same colors as the names of each state or the reference structures 3T3P (closed) and 2VDR (open). For clarity, water molecules as spheres and metal coordination bonds as red dashes are shown only for the second named structure in each image. Nitrogens and oxygens are shown in blue and red, respectively.