![Figure 1. Drp1-001 and -101 splice variants localize to the interphase MT cytoskeleton. (A) Shown is a domain diagram of Drp1 with an alignment of the central portion of the variable domain (VD) containing the second (red) and third (green) alternative exon from human, rat, and opossum (opos.). Black star and encircled “P” indicate critical residues and the SerCDK phosphorylation site, respectively. (B–D) HeLa cells expressing the indicated GFP-tagged Drp1 splice variants in place of endogenous Drp1 were analyzed for colocalization of the mitochondrial fission enzyme with β-tubulin by immunofluorescence. (B) Representative epifluorescence images of cells under basal conditions, after MT depolymerization (100 ng/ml nocodazole, 6 h), and after MT stabilization (10 µM taxol, 6 h) show that Drp1-101 follows the distribution of β-tubulin in different polymerization states. Drp1 colocalization with β-tubulin was quantified as the Pearson’s coefficient and is shown as a frequency distribution (C) and population means (D; means ± SEM [up]/SD [down] of ∼100 cells). *, P < 10−15 compared with all cytosolic splice variants.](https://cdn.rupress.org/rup/content_public/journal/jcb/201/7/10.1083_jcb.201210045/6/jcb_201210045_fig1.jpeg?Expires=1771701917&Signature=sFNpwK6uyC7Xs9hC8i8SEsPCf674IPwkoFKixdM8tzeM~R0dvpcdh~9pyvV5RaYR6ceA1c39Fp2VLmMKIcQ-CAM3QyR-ospPxMkon~sopOagOW4e-puroX5aC16SbmqEYm9q2fJQvONeUuXMpiA99OnZLvI4qMeWKU1Xa5mFrlEfga85lT3Dh5nsU7qVSgGkU9HNveM6RKfqowJdWx3eF7tAWvtzfJ1AJzVkgNdCE8czqPy2qap7u4rjOXGnp~73UHS~J0At8mAWCILCfV5muSzTV6f0DKQOiq89ACpWe6LAZTHAaRF2PVyAQBelprPtbnVN1A9WhNzBWPdJWz7OGA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Drp1-001 and -101 splice variants localize to the interphase MT cytoskeleton. (A) Shown is a domain diagram of Drp1 with an alignment of the central portion of the variable domain (VD) containing the second (red) and third (green) alternative exon from human, rat, and opossum (opos.). Black star and encircled “P” indicate critical residues and the SerCDK phosphorylation site, respectively. (B–D) HeLa cells expressing the indicated GFP-tagged Drp1 splice variants in place of endogenous Drp1 were analyzed for colocalization of the mitochondrial fission enzyme with β-tubulin by immunofluorescence. (B) Representative epifluorescence images of cells under basal conditions, after MT depolymerization (100 ng/ml nocodazole, 6 h), and after MT stabilization (10 µM taxol, 6 h) show that Drp1-101 follows the distribution of β-tubulin in different polymerization states. Drp1 colocalization with β-tubulin was quantified as the Pearson’s coefficient and is shown as a frequency distribution (C) and population means (D; means ± SEM [up]/SD [down] of ∼100 cells). *, P < 10−15 compared with all cytosolic splice variants.
