Repression of Yki does not mediate the function of Wts but rather provides negative feedback. (A–C) Control egg chambers at stage 8 (A), 9 (B), or 10 (C) labeled with phalloidin (red) to visualize the actin cytoskeleton, GFP (green) to mark the clones of cells generated using actin flipout GAL4, and DAPI (blue) to stain all nuclei. (D–I) Egg chambers with border cell clusters (arrows) that overexpress wild-type UAS-Yki at stage 8 (D), 9 (E), or 10 (F), or constitutively active UAS-Yki^S168A at stage 8 (G), 9 (H), or 10 (I) (all clones GFP positive). In all these Yki-expressing genotypes, border cell migration is accelerated and all the clusters migrate posteriorly toward the oocyte prematurely. (J) Quantification of the percentage of stage 9 border cell clusters that prematurely reach the oocyte (n > 20 for each genotype). Anterior is to the left in all panels. Insets show F-actin staining in border cell clusters. Bars, 50 µm (5 µm for insets).