Hif-1α controls cell dispersion in a Twist-dependent manner. (A–D) In situ hybridization against the neural crest marker Snail2. (A) Injection of ATGMOTwist into the prospective neural crest at the 16- and 32-cell stage embryo does not affect neural crest induction. Asterisk shows the injected side. (B–D) Injection of ATGMOTwist blocks neural crest migration. (E–S) Analysis of dispersion of cultured neural crest cells as described in Fig 6. (E–J) Nuclear labeling is shown at time 0 and after 3 h. (K–M) Delaunay triangulation shown for 3 h time to analyze the distance between neighbor cells. Color coded according to the area of the triangles after the indicate treatments. (N–P) Bright-field images after the indicated treatments. (Q–S) Membrane-GFP merged with nuclear-RFP to observe cell morphology. (T) Quantification of Delaunay triangulation for all the conditions analyzed. ***, P < 0.005. Errors bars represent standard deviation of three independent experiments, with a minimal of 60 cells analyzed for each condition. (U) Percentage of explants exhibiting cell dispersion.