Hif-1α promotes neural crest dispersion. Neural crest cells labeled with nuclear-RFP and membrane-GFP were cultured in vitro for 3 h, and cells dispersion was analyzed. (A–E) Nuclear labeling is shown at time 0 and after 3 h. (F–J) Delaunay triangulation shown for a 3-h time to analyze the distance between neighbor cells. Color coded according to the area of the triangles. (K–O) Bright-field images after the indicate treatments. (P–T) Membrane-GFP merged with nuclear-RFP to observe cell morphology, after the indicated treatments. (P) Quantification of Delaunay triangulation for all the conditions analyzed. ***, P < 0.005. Errors bars represent standard deviation, calculated from three independent experiments, with a minimal of 60 cells for each condition.