Hif-1α gain of function induces cell dispersion and inhibits chemotaxis. Chemotaxis was analyzed as described in Fig. 4 for each treatment as indicated at the top of the figure. (A–E) Time 0. (F–J) 3 h after culture. (K–O) Tracks of migrating cells. (P–T) Angles of migration for individual cells. (U) Cell velocity. (V) Persistence. (W) Chemotaxis index. Each experiment was performed at least three times, and ∼60 cells were analyzed for each treatment. ***, P < 0.005. Errors bars represent standard deviation.