Figure 7.
Figure 7. Morphology of MG132-treated WT and RIM1α KO synapses by cryo-ET. (A and C) Tomographic slices of MG132-treated WT (A) and RIM1α KO (C) synapses. PSD, postsynaptic density; SC, synaptic cleft; SV, synaptic vesicle; white arrowheads, tethers. Tomographic slices are 5.4 nm thick. Bars, 100 nm. B and D show corresponding 3D renderings of all vesicles analyzed (left) and of the AZ and proximal vesicles seen from the cytoplasmic side (right). AZ (gray), synaptic vesicles (yellow), tethers (blue), and connectors (red) are shown. For scale reference, mean vesicle diameter was 40.1 ± 5.4 nm (mean ± SD; no scale bars are shown because the image is rendered with 3D perspective). MG132-treated WT and RIM1α KO synapses were comparable in terms of proximal vesicle concentration and vesicle tethering to the AZ.

Morphology of MG132-treated WT and RIM1α KO synapses by cryo-ET. (A and C) Tomographic slices of MG132-treated WT (A) and RIM1α KO (C) synapses. PSD, postsynaptic density; SC, synaptic cleft; SV, synaptic vesicle; white arrowheads, tethers. Tomographic slices are 5.4 nm thick. Bars, 100 nm. B and D show corresponding 3D renderings of all vesicles analyzed (left) and of the AZ and proximal vesicles seen from the cytoplasmic side (right). AZ (gray), synaptic vesicles (yellow), tethers (blue), and connectors (red) are shown. For scale reference, mean vesicle diameter was 40.1 ± 5.4 nm (mean ± SD; no scale bars are shown because the image is rendered with 3D perspective). MG132-treated WT and RIM1α KO synapses were comparable in terms of proximal vesicle concentration and vesicle tethering to the AZ.

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