Figure 6.
Figure 6. Western blot analysis for ubiquitin and various presynaptic proteins. The double band detected for RIM1α corresponds to splice variants (see, e.g., Fig. 1 of Kaeser et al., 2008). MG132 induced an increase in the levels of RIM1α (WT), RIM1β (KO), and MUNC13 (WT and KO) and a smaller increase in RIM2 (KO) but not in other presynaptic proteins. Sample sizes (pairs of WT and KO littermates) are as follows: seven (RIM1 and MUNC13), six (RIM2), and three to four (ELKS, Liprin2, Liprin3, Rab3, synaptotagmin1, syntaxin1, SNAP25, VAMP2, and ubiquitin).

Western blot analysis for ubiquitin and various presynaptic proteins. The double band detected for RIM1α corresponds to splice variants (see, e.g., Fig. 1 of Kaeser et al., 2008). MG132 induced an increase in the levels of RIM1α (WT), RIM1β (KO), and MUNC13 (WT and KO) and a smaller increase in RIM2 (KO) but not in other presynaptic proteins. Sample sizes (pairs of WT and KO littermates) are as follows: seven (RIM1 and MUNC13), six (RIM2), and three to four (ELKS, Liprin2, Liprin3, Rab3, synaptotagmin1, syntaxin1, SNAP25, VAMP2, and ubiquitin).

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