Figure 2. Synaptic vesicle... | Rockefeller University Press

Figure 2.

$Figure 2. Synaptic vesicle concentration. For vesicles within 250 nm from the AZ, shown as the fraction of cytoplasmic volume occupied by vesicles, according to their distance to the AZ. (A) Mean vesicle concentration versus distance to the AZ. Error bars show SEMs. Proximal vesicle concentration was significantly reduced in RIM1α KO. (B–F) Individual vesicle concentration profiles for all synapses in WT, KO-altered, KO-aligned, WT + MG132, and KO + MG132 categories, respectively. Thicker dotted profiles represent means. Confidence values: *, P < 0.05; **, P < 0.01. Whereas all WT synapses showed a characteristic profile, RIM1α KO synapses were classified in two subpopulations (KO altered and KO aligned) according to the existence of vesicle concentration maxima within the proximal zone. In contrast, most MG132-treated RIM1α KO synapses showed a concentration profile comparable to WT. The numbers of animals, synapses, and vesicles analyzed for each category are shown in Table S1. SV, synaptic vesicle.$

Synaptic vesicle concentration. For vesicles within 250 nm from the AZ, shown as the fraction of cytoplasmic volume occupied by vesicles, according to their distance to the AZ. (A) Mean vesicle concentration versus distance to the AZ. Error bars show SEMs. Proximal vesicle concentration was significantly reduced in RIM1α KO. (B–F) Individual vesicle concentration profiles for all synapses in WT, KO-altered, KO-aligned, WT + MG132, and KO + MG132 categories, respectively. Thicker dotted profiles represent means. Confidence values: *, P < 0.05; **, P < 0.01. Whereas all WT synapses showed a characteristic profile, RIM1α KO synapses were classified in two subpopulations (KO altered and KO aligned) according to the existence of vesicle concentration maxima within the proximal zone. In contrast, most MG132-treated RIM1α KO synapses showed a concentration profile comparable to WT. The numbers of animals, synapses, and vesicles analyzed for each category are shown in Table S1. SV, synaptic vesicle.