Similar amounts of KT motors (CENP-E and dynein) accumulate at middle and peripheral KTs. (A) Examples of metaphase PtK1 cells immunostained for CENP-E (top, first column) or dynein (bottom, first column) and ACA (second column). The chromosomes were stained with DAPI. In the merged images, DAPI is shown in blue, ACA in red, and the motors in green. Bar, 5 µm. (B and C) Quantification of CENP-E (B) and dynein (C) at the KTs of middle versus peripheral KTs (n = 46 cells) Error bars indicate mean ± SEM.