Figure 3.
Figure 3. VL initiate closure of paracellular diapedesis gaps. (a) Dynamic epifluorescence (red) and TIRF (green) imaging of a paracellular diapedesis gap (yellow line) closure in an MVEC by a membrane protrusion (VL, arrowhead). Strong TIRF signal (i) and doubling of epifluorescence intensity (i–iii) demonstrate propagation as VL. n > 30. (b) Analysis of width and dynamics of VL. n > 35. (c) Percentage of three VL classes (i) and analysis of VL travel distances (ii). n > 100. (d) Dynamic imaging of mDsRed (red) and VE-cadherin-GFP (green) during gap closure. Cyan arrowheads indicate VL-mediated restoration of intercellular contacts followed by accumulation of VE-cadherin. (e) MVECs expressing mDsRed were imaged during gap closure (cyan arrowhead; i), fixed and stained for VE-cadherin and β-catenin (ii), and subjected to Pearson’s colocalization analysis (iii). n > 18. Bars, 5 µm. All values are mean ± SEM.

VL initiate closure of paracellular diapedesis gaps. (a) Dynamic epifluorescence (red) and TIRF (green) imaging of a paracellular diapedesis gap (yellow line) closure in an MVEC by a membrane protrusion (VL, arrowhead). Strong TIRF signal (i) and doubling of epifluorescence intensity (i–iii) demonstrate propagation as VL. n > 30. (b) Analysis of width and dynamics of VL. n > 35. (c) Percentage of three VL classes (i) and analysis of VL travel distances (ii). n > 100. (d) Dynamic imaging of mDsRed (red) and VE-cadherin-GFP (green) during gap closure. Cyan arrowheads indicate VL-mediated restoration of intercellular contacts followed by accumulation of VE-cadherin. (e) MVECs expressing mDsRed were imaged during gap closure (cyan arrowhead; i), fixed and stained for VE-cadherin and β-catenin (ii), and subjected to Pearson’s colocalization analysis (iii). n > 18. Bars, 5 µm. All values are mean ± SEM.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal