Figure 5.
Figure 5. Cdo is required for esophageal smooth muscle fascicle morphogenesis during pattering of the ME. (A) Longitudinal sections of P0, P7, and P14 Cdo+/− and Cdo−/− esophagi stained with antibodies to α-SMA and with DAPI. Micrographs of the LES and esophageal region just distal to the transition zone (Below TZ) are shown. Between P0 and P14, Cdo+/− smooth muscle fascicles changed their shape and orientation in a distal-to-proximal manner; this process was impaired in Cdo−/− esophagi. Bar, 50 µm. (B) Tracings of smooth muscle fascicles just proximal to the LES in P0, P7, and P14 Cdo+/− and Cdo−/− esophagi indicates the changes in shape and orientation. (C) Quantification of the total cross-sectional smooth muscle area in P7 and P14 Cdo+/− and Cdo−/− esophagi. (D) Quantification of the cross-sectional area of individual SMCs immediately distal to the TZ in Cdo+/− and Cdo−/− mice. Values are means ± SD, n = 3–5. *, P < 0.05; **, P < 0.001.

Cdo is required for esophageal smooth muscle fascicle morphogenesis during pattering of the ME. (A) Longitudinal sections of P0, P7, and P14 Cdo+/− and Cdo−/− esophagi stained with antibodies to α-SMA and with DAPI. Micrographs of the LES and esophageal region just distal to the transition zone (Below TZ) are shown. Between P0 and P14, Cdo+/− smooth muscle fascicles changed their shape and orientation in a distal-to-proximal manner; this process was impaired in Cdo−/− esophagi. Bar, 50 µm. (B) Tracings of smooth muscle fascicles just proximal to the LES in P0, P7, and P14 Cdo+/− and Cdo−/− esophagi indicates the changes in shape and orientation. (C) Quantification of the total cross-sectional smooth muscle area in P7 and P14 Cdo+/− and Cdo−/− esophagi. (D) Quantification of the cross-sectional area of individual SMCs immediately distal to the TZ in Cdo+/− and Cdo−/− mice. Values are means ± SD, n = 3–5. *, P < 0.05; **, P < 0.001.

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