Clusters of the EcDnΔ-UtrABD chimera are cis-interface independent. (a–b′) Immunostaining for the EcDnΔ-UtrABD (a, Utr) or its cis mutant, cis-EcDnΔ-UtrABD (b, cis-Utr), and actin filaments (a′ and b′, actin). The area indicated by the arrow is enlarged in the insets. Bars: (main images) 10 µm; (insets) 2.5 µm. (c) Dendra photoconversion assay of the clusters made of EcDnΔ-UtrABD (Δ-Utr) and cis-EcDnΔ-UtrABD (Cis-Δ-Utr) in comparison with those made of EcDnΔ-αABD (Δ-αABD). See Fig. 7 c for details. Note that UtrABD mediated formation of the giant cadherin clusters extending along the actin bundles. Inactivation of the cis-interface had little effect on the morphology of the clusters or their internal dynamics. The error bars represent SD (n = 30).