αABD cooperates with the cadherin cis-interface. (a–a′′) Immunostaining for the cis-EcDnΔ-αABD chimera (a) and for actin filaments (a′). Note that many cadherin clusters were still associated with the actin filaments (some are indicated by the arrows) and concentrated along the apical region of the cell–cell contacts. Bars: (main images) 20 µm; (insets) 2.5 µm. (b) Time-lapse imaging of the cis-EcDnΔ-αABD clusters (frames are indicated according to Video 6). The movement of one of the clusters is traced by the arrows. Note that the clusters are stable and exhibit basal to apical movement. Bar, 5 µm. (c) Dendra photoconversion assay of the clusters in cells expressing EcDnΔ-αABD (Δ-αABD), cis-EcDnΔ-αABD (Cis-Δ-αABD), and D1A/Cis-EcDnΔ-αABD (D1A/Cis-Δ-αABD). The graph shows changes in intensity of the red fluorescence in the individual junctions over time (in minutes) after the Dendra2 green to red photoconversion. The error bars represent SD (n = 30).