Figure 4. The D1A mutation impeded the... | Rockefeller University Press

Figure 4.

$Figure 4. The D1A mutation impeded the basal to apical movement of the EcDnΔ-αABD clusters. (a and b) The calcium-switch assay with the cells expressing EcDnΔ-αABD (a, α) and D1A-EcDnΔ-αABD (b, D1Aα). The cells immediately before the switch (0) and 5 min after the switch (5) are presented. Time-lapse images (numbering according to Videos 4 and 5) of the boxed regions in a and b are shown at the bottom. Note that the D1A mutation did not affect the initial assembly of the clusters but significantly reduced their movement. Arrows show a large motile (in a) and stationary (in b) clusters. Bars: (top) 20 µm; (bottom) 5 µm. (c) The path of the mean cluster (presented as a fraction of the basal to apical distance, assuming the entire distance for 100%; error bars are SDs) over 5 min after the calcium switch in cells expressing EcDnΔ-αABD (α), D1A-EcDnΔ-αABD (D1Aα), or in cells expressing EcDnΔ-αABD in the presence of 100 µM CK548 (CK548) or the low dose (25 nM) of cytochalasin D (CD), inhibitors of actin polymerization (see also Fig. 5).$

The D1A mutation impeded the basal to apical movement of the EcDnΔ-αABD clusters. (a and b) The calcium-switch assay with the cells expressing EcDnΔ-αABD (a, α) and D1A-EcDnΔ-αABD (b, D1Aα). The cells immediately before the switch (0) and 5 min after the switch (5) are presented. Time-lapse images (numbering according to Videos 4 and 5) of the boxed regions in a and b are shown at the bottom. Note that the D1A mutation did not affect the initial assembly of the clusters but significantly reduced their movement. Arrows show a large motile (in a) and stationary (in b) clusters. Bars: (top) 20 µm; (bottom) 5 µm. (c) The path of the mean cluster (presented as a fraction of the basal to apical distance, assuming the entire distance for 100%; error bars are SDs) over 5 min after the calcium switch in cells expressing EcDnΔ-αABD (α), D1A-EcDnΔ-αABD (D1Aα), or in cells expressing EcDnΔ-αABD in the presence of 100 µM CK548 (CK548) or the low dose (25 nM) of cytochalasin D (CD), inhibitors of actin polymerization (see also Fig. 5).