Distribution and dynamics of the EcDnΔ-αABD clusters. (a–b′′) Double-label immunofluorescence microscopy of A431D cells expressing EcDnΔ-αABD (a, α) or D1A-EcDnΔ-αABD (b, D1Aα) chimeras. Cells were stained for chimeras using the anticadherin antibody (a and b) and for F-actin using Alexa Fluor 555 phalloidin (a′ and b′, actin). The corresponding images are merged in a′′ and b′′. Note that most of the cadherin clusters (two of the subapical and one of the basal clusters are indicated by arrowheads and an arrow in the insets) associated with actin bundles. Bars: (main images) 40 µm; (insets) 5 µm. (c) Time-lapse series (right) of EcDnΔ-αABD clusters (frames are indicated according to Video 3) present in the boxed region of the left image. Arrows of the same color indicate the same clusters in consequent frames. Note that the clusters produced by this chimera had a much longer lifetime than the tailless clusters and exhibited directional movements. Bars: (left) 10 µm; (right) 5 µm.