Dendra diffusion assay with cells expressing EcDnΔ, D1A-EcDnΔ, and Cis-EcDnΔ. (a) The circular areas (diameter = 2.5 µm) in the selected cell–cell contact of EcDnΔ cells, which express relatively high level of the mutants (−3), was photoconverted from green to red fluorescence (the photoconverted area is circled in the red channel image). The region was imaged at 3 and 23 s (0 and 20) after photoconversion in both green and red channels. Image acquisition time was 1 s. Bar, 10 µm. (b) The experiment shown in panel a was repeated 10 times with each of the indicated cell lines. Error bars indicate SDs. The graph shows the expansion of the red fluorescence area.