Integration of proteome abundance and subcellular organization enables characterization of the dynamics of organelle morphology and composition under stress. (a) Hierarchical clustering of mean log₂ ratio of the change in abundance of proteins in each organelle under stress relative to SD. (b) Representative images of nucleolus proteins in SD or during growth in starvation medium. Colocalization with a nuclear mCherry (NLS) allows visualization of the massive shrinkage of this compartment relative to the nucleus under these conditions. (c) Representative images of cell periphery proteins that are expressed only under starvation and cause an increase in the protein levels in this organelle. (d) Representative images of mitochondrial proteins tagged with GFP coexpressed with a matrix-targeted dsRed (MTS-dsRed) during growth in SD or H₂O₂ treatment. During stress these proteins become localized to the vacuole lumen or membrane (as can be seen by the corresponding bright-field image) concomitantly with presence of the mitochondrial dsRed signal in the vacuole. (e) Mitochondrial fragmentation occurs when cells are exposed to medium containing 1 mM H₂O₂. This fragmentation is blocked in the absence of the fission machinery proteins Fis1, Dnm1, or Mdv1. (f) Fragmentation seems to be required for growth during oxidative stress as loss of fission capacity reduces fitness of cells under 1 mM H₂O₂ relative to control (YEPD). Bars, 5 µm.