Figure 2. A bet-hedging strategy in... | Rockefeller University Press

Figure 2.

$Figure 2. A bet-hedging strategy in yeast underlies prolonged survival under starvation. (a) Flow cytometry dot plots show the transition of unimodal distributed population of Pre3-GFP in SD (left) into two distinct subpopulations after 24 h of starvation (right; a.u., arbitrary units). High and low fractions (marked with broken ellipses on the right plot) were sorted accordingly. Microscopic analysis of the two sorted subpopulations demonstrates distinct morphologies. Bars, 5 µm. (b) Growth of the two subpopulations in SD immediately after sorting (24 h starvation, left) versus 4 d after sorting (120 h starvation, right) of both Pre3-GFP and Ssb1-GFP (OD). Each growth well contained 100,000 cells at the starting point. These measurements are the representative means of two repeats; error bars indicate standard deviation in black.$

A bet-hedging strategy in yeast underlies prolonged survival under starvation. (a) Flow cytometry dot plots show the transition of unimodal distributed population of Pre3-GFP in SD (left) into two distinct subpopulations after 24 h of starvation (right; a.u., arbitrary units). High and low fractions (marked with broken ellipses on the right plot) were sorted accordingly. Microscopic analysis of the two sorted subpopulations demonstrates distinct morphologies. Bars, 5 µm. (b) Growth of the two subpopulations in SD immediately after sorting (24 h starvation, left) versus 4 d after sorting (120 h starvation, right) of both Pre3-GFP and Ssb1-GFP (OD). Each growth well contained 100,000 cells at the starting point. These measurements are the representative means of two repeats; error bars indicate standard deviation in black.