Figure 3. Msn reduces integrin-based... | Rockefeller University Press

Figure 3.

$Figure 3. Msn reduces integrin-based adhesion. (A) Basal views of msn mosaic epithelia stained with antibodies against α-PS1 (maximum projection) or β-PS show increased integrin levels at the basal surfaces of msn cells. GFP marks wild-type cells. (B) A clone of cells expressing an activated form of Msn (green) shows reduced integrins at the basal surface. (C) Maximum projection of Vkg-GFP in the BM. Loss of Msn disrupts BM structure in a cell-autonomous manner, and BM structure is restored by introducing one copy of a loss-of-function β-PS mutation (mysXG43/+; msn-RNAi). Bars are as indicated in the figure. (D) Removing one copy of β-PS also rescues the defect in follicle cell migration seen in msn-RNAi, whereas removing one copy of β-PS alone or overexpressing Msn (UAS-Msn) increases the migration rate. Graph shows migration rates of individual stage 6–7 egg chambers for each condition. Numbers indicate fraction of egg chambers that showed follicle cell migration. The data shown are from a single representative experiment out of two repeats. Each experimental condition involves egg chambers dissected from >10 female flies, imaged over multiple days.$

Msn reduces integrin-based adhesion. (A) Basal views of msn mosaic epithelia stained with antibodies against α-PS1 (maximum projection) or β-PS show increased integrin levels at the basal surfaces of msn cells. GFP marks wild-type cells. (B) A clone of cells expressing an activated form of Msn (green) shows reduced integrins at the basal surface. (C) Maximum projection of Vkg-GFP in the BM. Loss of Msn disrupts BM structure in a cell-autonomous manner, and BM structure is restored by introducing one copy of a loss-of-function β-PS mutation (mys^XG43/+; msn-RNAi). Bars are as indicated in the figure. (D) Removing one copy of β-PS also rescues the defect in follicle cell migration seen in msn-RNAi, whereas removing one copy of β-PS alone or overexpressing Msn (UAS-Msn) increases the migration rate. Graph shows migration rates of individual stage 6–7 egg chambers for each condition. Numbers indicate fraction of egg chambers that showed follicle cell migration. The data shown are from a single representative experiment out of two repeats. Each experimental condition involves egg chambers dissected from >10 female flies, imaged over multiple days.